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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in several tumors, which leads to chromosome instability, and thus might contribute to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical role in promoting mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two further splice variants have been noted but detailed nucleotide sequence facts is just not however out there.
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Blocking peptides are peptides that bind precisely towards the goal antibody and block antibody binding. These peptide commonly includes the epitope identified by the antibody.
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This manual outlines further doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines further probable optimization of mobile and click here immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to your epitope within the concentrate on protein. By comparing the staining with the blocked antibody vs . the antibody alone, one can see which staining is certain.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin family members, whose customers are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which lead to your temporal coordination of each and every mitotic occasion. Cyclin E varieties a posh with and functions as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S stage boundary and is also degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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Clicking the images or links will redirect you to definitely a website hosted by BenchSci that provides 3rd-celebration scientific articles. Neither the information nor the BenchSci technological know-how and procedures for assortment have been evaluated by us; we're furnishing them as-is and without having warranty of any kind, which include for use or application from the Thermo Fisher Scientific items offered.